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T cell receptor genes in an alloreactive CTL clone: implications for rearrangement and germline diversity of variable gene segments.
Author(s) -
Chou H.S.,
Behlke M.A.,
Godambe S.A.,
Russell J.H.,
Brooks C.G.,
Loh D.Y.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04478.x
Subject(s) - biology , ctl* , germline , gene , genetics , gene rearrangement , clone (java method) , t cell receptor , t cell , cytotoxic t cell , immune system , in vitro
Both cDNA and genomic clones of the T cell receptor (TCR) alpha‐ and beta‐chain genes of the alloreactive cytotoxic T lymphocyte (CTL) clone F3 were examined. Two distinct rearrangement events, one functional and one non‐functional, were found for both the alpha and beta loci. Thus only a single functional TCR alpha beta heterodimer could be defined, consistent with allelic exclusion in the TCR genes. The V alpha gene employed by F3 is part of a six‐member V alpha subfamily. Genomic clones containing each member of this subfamily were isolated and the V alpha nucleotide sequences determined. Five of these six genes are functional; these genes differ from each other by 7‐14% at the amino acid level. A single dominant hypervariable region was defined within this subfamily, in contrast to the pattern of variability seen between V alpha genes in general.