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Identification and characterization of the protein encoded by the human c‐myb proto‐oncogene.
Author(s) -
Klempnauer K.H.,
Bonifer C.,
Sippel A.E.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04443.x
Subject(s) - biology , identification (biology) , myb , computational biology , genetics , microbiology and biotechnology , gene , transcription factor , botany
We have identified the product of the human c‐myb proto‐oncogene as a 80,000‐Mr protein, p80c‐myb, by using polyclonal and monoclonal antibodies raised against a bacterially synthesized polypeptide from the amino terminus of the viral myb protein. p80c‐myb shares at least two distinct antigenic sites with the amino terminal region of the v‐myb protein. p80c‐myb is found only in hematopoietic cells or in cells that contain amplified c‐myb genes. Like the chicken myb proteins, p80c‐myb is a nuclear DNA‐binding protein that is predominantly associated with chromatin and exhibits a short half‐life of approximately 1 hour.