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Studies on the expression of an H‐2K/human growth hormone fusion gene in giant transgenic mice.
Author(s) -
Morello D.,
Moore G.,
Salmon A.M.,
Yaniv M.,
Babinet C.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04439.x
Subject(s) - biology , gene , transgene , fusion gene , microbiology and biotechnology , growth hormone , genetics , human growth hormone , gene expression , hormone , biochemistry
Transgenic mice carrying the H‐2K/human growth hormone (hGH) fusion gene were produced by microinjecting into the pronucleus of fertilized eggs DNA molecules containing 2 kb of the 5′ flanking sequences (including promoter) of the class I H‐2Kb gene joined to the coding sequences of the hGH gene. Thirteen transgenic mice were obtained which all contained detectable levels of hGH hormone in their blood. Nine grew larger than their control litter‐mates. Endogenous H‐2Kb and exogenous hGH mRNA levels were analysed by S1 nuclease digestion experiments. hGH transcripts were found in all the tissues examined and the pattern of expression paralleled that of endogenous H‐2K gene expression, being high in liver and lymphoid organs and low in muscle and brain. Thus 2 kb of the 5′ promoter/regulatory region of the H‐2K gene are sufficient to ensure regulated expression of hGH in transgenic mice. This promoter may therefore be of use to target the expression of different exogenous genes in most tissues of transgenic mice and to study the biological role of the corresponding proteins in different cellular environments.