Premium
Inhibition of N‐linked oligosaccharide trimming mannosidases blocks human B cell development.
Author(s) -
Tulp A.,
Barnhoorn M.,
Bause E.,
Ploegh H.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04427.x
Subject(s) - biology , oligosaccharide , trimming , microbiology and biotechnology , biochemistry , computer science , operating system
Deoxymannojirimycin (dMM) or swainsonine (SW), which block conversion of high‐mannose to complex‐type N‐linked glycans, strongly inhibited the production of immunoglobulin (Ig) when added to cultures of human lymphocytes together with the polyclonal B cell activators pokeweed mitogen (PWM) and Staphylococcus aureus (SAC). To obtain the inhibitory effect, inhibitor had to be present during the first 36 h of culture. Addition at later timepoints was less effective and showed that neither inhibitor interfered with rate of production or secretion of Ig as such. Viability and proliferation of the lymphocytes, as defined by cell number and rate of DNA synthesis, were not influenced by the presence of dMM or SW, and no changes in the relative number of helper (T4+) or suppressor (T8+) cells were observed. Thus, for normal differentiation of human B lymphocytes into Ig secreting (plasma) cells in response to PWM and SAC, conversion of high‐mannose to complex N‐linked glycans is essential.