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The nucleotide sequence of an infectious clone of the geminivirus beet curly top virus
Author(s) -
Stanley J.,
Markham P.G.,
Callis R.J.,
Pinner M.S.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04424.x
Subject(s) - biology , geminiviridae , virology , genetics , genome , leafhopper , plant virus , begomovirus , virus , nucleic acid sequence , dna virus , dna , gene , hemiptera , botany
A number of infectious clones of a Californian isolate of the leafhopper‐transmitted geminivirus beet curly top virus (BCTV) have been constructed from virus‐specific double‐stranded DNA isolated from infected Beta vulgaris and used to demonstrate a single component genome. The nucleotide sequence of one infectious clone has been determined (2993 nucleotides). Comparison with other geminiviruses has shown that the organisation of the genome closely resembles DNA 1 of the whitefly‐transmitted members. The four conserved coding regions of DNA 1 have highly homologous counterparts in BCTV with the exception of the putative coat protein which is more closely related to those of the leafhopper‐transmitted geminiviruses suggesting a strong interrelationship between coat protein and insect vector. A BCTV component equivalent to DNA 2 is not required for virus infection or transmission and has not been isolated from infected plants.