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Two RNA species co‐purify with RNase P from the fission yeast Schizosaccharomyces pombe.
Author(s) -
Krupp G.,
Cherayil B.,
Frendewey D.,
Nishikawa S.,
Söll D.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04413.x
Subject(s) - schizosaccharomyces pombe , biology , schizosaccharomyces , rnase p , fission , yeast , rna , exosome complex , genetics , saccharomyces cerevisiae , gene , physics , quantum mechanics , neutron
RNase P activity from Schizosaccharomyces pombe co‐purifies with two RNA species. These RNAs are associated with enzyme activity as judged by titrated micrococcal nuclease inactivation experiments. The two RNAs, K1‐ and K2‐RNA, are 285 and 270 nucleotides long, respectively. Both RNAs are transcribed from one gene, present in a single copy in the haploid genome. The primary and a secondary structure of K RNAs have been determined and compared with M1 RNA, their counterpart from Escherichia coli. Very limited sequence homology was observed, and this agrees with the finding that no cross‐hybridization with M1 RNA can be detected in a Southern analysis with yeast genomic DNA. However, the secondary structures of K RNA and M1 RNA show the same basic organization and one conserved local motif, the sequence GUG‐‐AGGPu in an exposed hairpin loop.