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Idiotypic selection of an antibody mutant with changed hapten binding specificity, resulting from a point mutation in position 50 of the heavy chain.
Author(s) -
Brüggemann M.,
Müller H.J.,
Burger C.,
Rajewsky K.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04397.x
Subject(s) - hapten , mutant , point mutation , microbiology and biotechnology , biology , mutation , antibody , idiotype , stereochemistry , genetics , gene , monoclonal antibody , chemistry
Somatic mutation occurs at a low rate in the rearranged antibody V region genes of the hybridoma line B1‐8. delta 1 which expresses an antibody with specificity for the hapten 4‐hydroxy‐3‐nitro‐5‐iodo‐phenylacetyl (NIP). A mutant was selected which had lost a binding site‐related idiotope but retained most of its other idiotypic determinants. The mutant had concomitantly lost NIP binding and acquired specificity for dinitrophenylated bovine serum albumin. It carried a single point mutation in position 50 of the heavy chain, resulting in the replacement of an arginine by a glycine.