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Synthetic lac operator mediates repression through lac repressor when introduced upstream and downstream from lac promoter.
Author(s) -
Besse M.,
WilckenBergmann B.,
MüllerHill B.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04370.x
Subject(s) - biology , lac repressor , repressor , lac operon , psychological repression , operator (biology) , upstream (networking) , downstream (manufacturing) , genetics , microbiology and biotechnology , gene , plasmid , gene expression , computer network , operations management , computer science , economics
Plasmids were constructed which carry a synthetic lac operator at various distances from the lac promoter. They were tested in vivo for function in the presence and absence of lac repressor. We found significant repression when the lac operator is situated at the 3′ end of the lac I gene or at the 5′ end of the lac Z gene. When lac operators are inserted at both sites, we found a greater than 150‐fold repression. The complex between lac repressor and DNA carrying these two lac operators is exceedingly stable in vitro suggesting that one tetrameric lac repressor may bind to both lac operators.