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Regulatory elements involved in Drosophila Adh gene expression are conserved in divergent species and separate elements mediate expression in different tissues.
Author(s) -
Fischer J.A.,
Maniatis T.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04357.x
Subject(s) - biology , drosophila melanogaster , gene , melanogaster , genetics , promoter , locus (genetics) , alcohol dehydrogenase , gene expression , conserved sequence , mutant , regulatory sequence , genome , enzyme , biochemistry , peptide sequence
Alcohol dehydrogenase (ADH) is expressed in a complex temporal and spatial pattern from tandem promoters (proximal and distal) in Drosophila melanogaster, and from two closely linked genes (Adh‐1 and Adh‐2) in D. mulleri. The expression patterns of Adh‐1 and the proximal promoter, and Adh‐2 and the distal promoter are similar, but not identical. We show that the mulleri Adh genes are appropriately expressed when introduced into the melanogaster genome, indicating that the cis‐ and trans‐acting elements which regulate the corresponding promoters are functionally equivalent in the two species. By analyzing the expression of in vitro generated mutants of the mulleri Adh locus, we define at least three regulatory regions of the mulleri Adh genes and show that different control elements mediate the expression of Adh in different tissues.