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Nucleotide sequence of the coding region of the mouse N‐myc gene.
Author(s) -
Taya Y.,
Mizusawa S.,
Nishimura S.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04349.x
Subject(s) - biology , gene , coding region , intron , exon , genetics , nucleic acid sequence , microbiology and biotechnology , peptide sequence , genomic dna , sequence (biology)
A genomic clone for the mouse N‐myc gene was isolated and the total nucleotide sequence (4807 bp) of the two coding exons and an intron located between them was determined. The amino acid sequence of the N‐myc protein was deduced from the DNA sequence. This protein is composed of 462 amino acids, slightly larger than human and mouse c‐myc proteins, and is rich in proline like the c‐myc protein. Comparison of the amino acid sequences of the mouse N‐myc and c‐myc proteins showed that conserved sequences are located in eight regions: four regions are in the N‐terminal half of the N‐myc protein and are separated from each other by regions poorly homologous to those of the c‐myc protein, and the four others are located in the C‐terminal half, throughout which certain homology exists. A remarkable sequence containing 13 successive acidic amino acids is present in one of the conserved regions located in the middle of the N‐myc protein.