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Modulation of urokinase plasminogen activator gene expression during the transition from quiescent to proliferative state in normal mouse cells.
Author(s) -
Grimaldi G.,
Di Fiore P.,
Locatelli E.K.,
Falco J.,
Blasi F.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04295.x
Subject(s) - activator (genetics) , gene , biology , microbiology and biotechnology , genetics
We have investigated the regulation of urokinase (u‐PA) mRNA in quiescent mouse fibroblasts and keratinocytes stimulated to divide by the addition of serum or epidermal growth factor (EGF), respectively. Serum stimulation of quiescent fibroblasts (BALB/c 3T3 or Swiss 3T3) results in an early and transient increase of u‐PA mRNA level, which precedes by several hours the onset of DNA synthesis. A similar response is elicited by EGF stimulation of quiescent keratinocytes. The increase of u‐PA mRNA parallels that of c‐myc mRNA, does not require protein synthesis and is at least in part due to increase in template activity of the u‐PA gene. Induction of terminal differentiation of mouse keratinocytes results in a decrease of u‐PA mRNA which parallels the decrease of thymidine incorporation. In conclusion, variation in the level of u‐PA mRNA is seen during G0/G1 transition and correlates with the proliferative state of these normal mouse cells.