Premium
Molecular cloning of the c locus of Zea mays : a locus regulating the anthocyanin pathway
Author(s) -
PazAres Javier,
Wienand Udo,
Peterson Peter A.,
Saedler Heinz
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04291.x
Subject(s) - locus (genetics) , biology , transposable element , genetics , clone (java method) , microbiology and biotechnology , molecular cloning , mutant , gene , peptide sequence
The c locus of Zea mays , involved in the regulation of anthocyanin biosynthesis, has been cloned by transposon tagging. A clone (#18En) containing a full size En1 element was initially isolated from the En element‐induced mutable allele c‐m668655 . Sequences of clone #18En flanking the En1 element were used to clone other c mutants, whose structure was predicted genetically. Clone #23En (isolated from c‐m668613 ) contained a full size En1 element, clone #3Ds (isolated from c‐m2 ) a Ds element and clone #5 (isolated from c +) had no element on the cloned fragment. From these data we conclude that the clones obtained contain at least part of the c locus. Preliminary data on transcript analysis using a 1‐kb DNA fragment from wild‐type clone #5 showed that at least three transcripts are encoded by that part of the locus, indicating that c is a complex locus.