Premium
Activation of the pp60c‐src kinase during differentiation of monomyelocytic cells in vitro.
Author(s) -
Barnekow A.,
Gessler M.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04270.x
Subject(s) - biology , rous sarcoma virus , proto oncogene tyrosine protein kinase src , tyrosine kinase , kinase , cellular differentiation , in vitro , cell culture , microbiology and biotechnology , gene , biochemistry , signal transduction , genetics
The proto‐oncogene c‐src, the cellular homolog of the Rous sarcoma virus (RSV) transforming gene v‐src, is expressed in a tissue‐specific and age‐dependent manner. Its physiological function, although still unknown, appears to be more closely related to differentiation processes than to proliferation processes. To obtain more information about the physiological role of the c‐src gene in cells, we have studied differentiation‐dependent alterations using the human HL‐60 leukaemia cell line as a model system. Induction of monocytic and granulocytic differentiation of HL‐60 cells by 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) and dimethylsulfoxide (DMSO) is associated with an activation of the pp60c‐src tyrosine kinase, but not with increased c‐src gene expression. Control experiments exclude an interaction of TPA and DMSO themselves with the pp60c‐src kinase.