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Regulated expression of repetitive sequences including the identifier sequence during myotube formation in culture.
Author(s) -
Herget T.,
Reich M.,
Stüber K.,
StarzinskiPowitz A.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04264.x
Subject(s) - biology , sequence (biology) , genetics , repeated sequence , computational biology , expression (computer science) , microbiology and biotechnology , gene , genome , computer science , programming language
We have isolated and characterized a cDNA of 1183 bp, pL6‐411, from rat L6 muscle cells. This cDNA contains repetitive sequences ‐ including two inverted copies of the previously described identifier sequence ‐ as shown by sequence analysis. Repetitive sequences from pL6‐411 characterize a family of RNAs which is specifically induced during L6 myotube formation. Another part of the pL6‐411 sequence, existing at low‐copy number per haploid rat genome, hybridized to two RNAs of 5 kb and 2 kb from L6 myoblasts as well as from L6 myotubes. A third pL6‐411‐related RNA of 150 bases was detected which hybridized with the repetitive sequence but did not hybridize with the low‐copy number part of pL6‐411. It appears that the ‘identifier’ sequence in this population of small RNAs is complementary to one of the ‘identifier’ copies in the pL6‐411‐related RNA. Finally, we identified on cDNA pL6‐411 the recognition site for the TGGCA‐binding protein and in both orientations a total of four putative promoters for RNA polymerase III.