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Variable DNA splicing sites of a mitochondrial intron: relationship to the senescence process in Podospora
Author(s) -
Belcour Léon,
Vierny Corinne
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04254.x
Subject(s) - biology , podospora anserina , mitochondrial dna , intron , rna splicing , genetics , senescence , dna , gene , microbiology and biotechnology , evolutionary biology , mutant , rna
The unavoidable phenomenon of senescence in Podospora was previously shown to be correlated with the presence of a senescence‐specific DNA originating from amplification of some regions of the mitochondrial chromosome. The most frequently amplified region (α) corresponds to the first intron of the gene coding for subunit one of cytochrome oxidase. Eleven long‐lived mitochondrial mutants were isolated. Here we report sequencing experiments that show that three of them are deleted for most of intron α and for a few base pairs belonging to the upstream adjacent exon. We also report an analysis of the residual mitochondrial DNA associated with amplification of senescence‐specific DNA α which allows us to identify, in senescent cultures, mitochondrial chromosomes lacking sequence α. These results taken together suggest that excision of intron α from the mitochondrial DNA occurs systematically during the aging process in Podospora . They furthermore provide the first example of inaccurate intron excision at the DNA level.