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The immunoglobulin heavy‐chain B‐lymphocyte enhancer efficiently stimulates transcription in non‐lymphoid cells.
Author(s) -
Wasylyk C.,
Wasylyk B.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04246.x
Subject(s) - biology , immunoglobulin heavy chain , enhancer , antibody , lymphocyte , transcription (linguistics) , microbiology and biotechnology , immunoglobulin light chain , lymphatic system , immunoglobulin m , transcription factor , immunoglobulin g , immunology , genetics , gene , linguistics , philosophy
The mouse immunoglobulin heavy‐chain (IgH) B‐lymphocyte enhancer stimulates transcription from heterologous promoters 20‐ to 40‐fold when transfected into several non‐lymphoid cell lines. Stimulation in B‐lymphocyte melanoma cell‐lines is only about 5‐‐10 times better. A central sequence is equally active in both cell types, whilst flanking sequences, on either side of the common enhancer sequences, specifically stimulate transcription in myeloma cells. These results suggest that there are factors in non‐lymphoid cells that can interact with the IgH enhancer to stimulate transcription.