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Transcription and expression of zein sequences in yeast under natural plant or yeast promoters
Author(s) -
Coraggio I.,
Compagno C.,
Martegani E.,
Ranzi B.M.,
Sala E.,
Alberghina L.,
Viotti A.
Publication year - 1986
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1986.tb04234.x
Subject(s) - biology , yeast , promoter , humanities , transcription (linguistics) , gene , genetics , gene expression , philosophy , linguistics
Maize genomic fragments containing the regulatory and coding regions of a zein gene for a low size class 23‐kd protein have been inserted in an interspecific Escherichia coli‐Saccharomyces cerevisiae expression vector in different constructions. The presence of the inducible GAL1‐10 upstream activation site (UAS) allows us to regulate differentially by carbon sources the transcription of the zein gene both under the plant promoter and under the yeast CYC‐1 promoter. We found that the zein promoter region is properly recognized at the correct transcription start, while different termination points occur during transcription. The yeast UAS was also shown to function as a typical eukaryotic enhancer regardless of its distance or orientation with respect to the plant promoter. Yeast cells transformed by a plasmid containing a zein sequence fused to a short piece of the CYC‐1 gene produced a fused polypeptide, of expected mol. wt, in variable amount from 0.2 to 5% depending on the growth phase conditions.