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Anti‐Z‐DNA antibody binding can stabilize Z‐DNA in relaxed and linear plasmids under physiological conditions.
Author(s) -
Lafer E.M.,
Sousa R.,
Rich A.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb04131.x
Subject(s) - biology , plasmid , dna , genetics , microbiology and biotechnology , computational biology
It is shown that anti‐Z‐DNA antibody binding can stabilize sequences of d(CG/GC)n and d(CA/GT)n in the Z‐DNA conformation in a plasmid in the complete absence of supercoiling. This effect is quantitated by using antibody preparations of different affinities and varying concentrations. The d(CG/GC)n sequence can be stabilized under physiological conditions. This is the first demonstration that a region of Z‐DNA can be stabilized by protein binding in a completely relaxed plasmid under physiological conditions. The antibody‐Z‐DNA complex in the relaxed plasmid is shown to be an equilibrium state and not a long‐lived kinetic intermediate since specific binding of the antibody to linearized plasmids containing Z‐forming sequences is observed.