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Control of gene expression in P2‐related coliphages: the in vitro transcription pattern of coliphage 186.
Author(s) -
Pritchard M.,
Egan J.B.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb04123.x
Subject(s) - biology , coliphage , transcription (linguistics) , gene , gene expression , in vitro , genetics , bacteriophage , microbiology and biotechnology , escherichia coli , linguistics , philosophy
Transcription in vitro of coliphage 186 DNA generated four transcripts. The most abundant transcript was that of the late control gene B and an equivalent transcript was identified for the closely related phage P2. A second transcript was from the rightward promoter at 75% and predicted to be under CI repressor control. The remaining two transcripts initiated from the one promoter located at 95% and are apparently under LexA control in vivo. The significance of these transcripts is discussed in relation to coliphage 186.