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In vivo transfer of genetic information between gram‐positive and gram‐negative bacteria.
Author(s) -
TrieuCuot P.,
Gerbaud G.,
Lambert T.,
Courvalin P.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb04120.x
Subject(s) - biology , gram , gram negative bacteria , bacteria , gram positive bacteria , in vivo , microbiology and biotechnology , genetics , escherichia coli , gene
A 1427‐bp DNA fragment containing the kanamycin resistance gene, aphA‐3, of plasmid pIP1433 from Campylobacter coli was inserted into a shuttle vector. Full expression of aphA‐3 was obtained in Bacillus subtilis and in Escherichia coli. This DNA fragment was sequenced in its entirety and the starting point for aphA‐3 transcription in B. subtilis, C. coli and E. coli was determined by S1 nuclease mapping. The sequence of the promoter consists of the hexanucleotides TTGACA and TATAAT, with a spacing of 17 bp. The nucleotide sequence of the aphA‐3 gene from C. coli and from the streptococcal plasmid pJH1 are identical whereas they differ by two substitutions and deletion of a codon from that cloned from the staphylococcal plasmid pSH2. These results indicate a recent extension of the resistant gene pool of Gram‐positive cocci to Gram‐negative bacilli. From an analysis of the DNA sequences surrounding the promoter region, we concluded that the DNA fragment containing the aphA‐3 gene in plasmid pJH1 has evolved by deletions from a sequence similar to that found in plasmid pIP1433.