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Cloning of opal suppressor tRNA genes of a filamentous fungus reveals two tRNASerUGA genes with unexpected structural differences.
Author(s) -
Debuchy R.,
Brygoo Y.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb04116.x
Subject(s) - biology , podospora anserina , gene , genetics , neurospora , cloning (programming) , transfer rna , schizosaccharomyces pombe , sequence (biology) , molecular cloning , mutant , rna , peptide sequence , neurospora crassa , computer science , programming language
The informational suppressors su4‐1 and su8‐1 of Podospora anserina were isolated by transformation of Schizosaccharomyces pombe UGA mutants. The DNA sequence revealed that they were opal (UGA) suppressor tRNAs. Wild‐type alleles were also isolated by hybridization. The DNA sequence showed that they both encode species of tRNASerUGA. The gene SU8 has an 18‐bp intervening sequence and its primary sequence is very different from that of SU4.