Premium
Transposon Tn554: complete nucleotide sequence and isolation of transposition‐defective and antibiotic‐sensitive mutants.
Author(s) -
Murphy E.,
Huwyler L.,
Freire Bastos M. do C.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb04089.x
Subject(s) - transposable element , biology , genetics , transposition (logic) , nucleic acid sequence , mutant , orfs , open reading frame , transposon mutagenesis , gene , peptide sequence , philosophy , linguistics
The complete nucleotide sequence of the Staphylococcus aureus transposon Tn554, which encodes resistance to erythromycin and spectinomycin, was determined by the dideoxy chain termination method. The transposon was found to be 6691 bp in length and to contain six open reading frames of greater than 125 amino acids. Small insertion and deletion mutations were obtained in each of these by in vitro mutagenesis at restriction endonuclease cleavage sites and the mutants characterized with respect to transposition functions and antibiotic resistance markers. Three of the reading frames, designated tnpA, tnpB and tnpC, encode functions that are required for transposition of Tn554; genetic analysis indicated that these three genes define distinct complementation groups of transposition‐defective mutants. Two of the open reading frames correspond to the resistance determinants spc and ermA, the sixth, designated ORF, has no known function. Tn554‐specific peptides corresponding to tnpA, and spc were identified in a coupled transcription‐translation system in vitro.