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Priming immunization against cholera toxin and E. coli heat‐labile toxin by a cholera toxin short peptide‐beta‐galactosidase hybrid synthesized in E. coli.
Author(s) -
Jacob C.O.,
Leitner M.,
Zamir A.,
Salomon D.,
Ar R.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb04086.x
Subject(s) - cholera toxin , toxin , biology , cholera , cholera vaccine , microbiology and biotechnology , enterotoxigenic escherichia coli , diphtheria toxin , peptide , immunogenicity , virology , vibrio cholerae , toxoid , epitope , escherichia coli , antibody , immunization , biochemistry , enterotoxin , immunology , bacteria , genetics , gene
A synthetic oligodeoxynucleotide encoding for a small peptide was employed for the expression of this peptide in a form suitable for immunization. The encoded peptide, namely, the region 50‐64 of the B subunit of cholera toxin (CTP3), had previously been identified as a relevant epitope of cholera toxin. Thus, multiple immunizations with its conjugate to a protein carrier led to an efficient neutralizing response against native cholera toxin. Immunization with the resulting fusion protein of CTP3 and beta‐galactosidase, followed by a booster injection of a sub‐immunizing amount (1 microgram) of cholera toxin, led to a substantial level of neutralizing antibodies against both cholera toxin and the heat‐labile toxin of Escherichia coli.