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The SV40 enhancer influences viral late transcription in vitro and in vivo but not on replicating templates.
Author(s) -
Shaw P.E.,
Bohmann D.,
Sergeant A.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb04073.x
Subject(s) - enhancer , transcription (linguistics) , biology , in vivo , in vitro , microbiology and biotechnology , transcription factor , genetics , gene , linguistics , philosophy
We have examined transcription from the SV40 late promoter in vitro and in vivo. In HeLa whole cell extracts, late transcription is efficient in the absence of T antigen but is impaired by enhancer specific point mutations. In vivo, when replication is prevented, transcription from the late promoter requires T antigen as well as a functional enhancer. However, enhancer sequences fail to potentiate late transcription from replicating templates although, under such conditions, enhancer binding factors do not become limiting. It appears that the SV40 late transcription unit is refractory to enhancer‐mediated activation when it is located on a replicating template.