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Photoregulated expression of a pea rbc S gene in leaves of transgenic plants
Author(s) -
Nagy F.,
Morelli G.,
Fraley R.T.,
Rogers S.G.,
Chua N.H.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb04046.x
Subject(s) - biology , petunia , nicotiana tabacum , transgene , transformation (genetics) , gene , genetically modified crops , genetics , mutant , expression vector , regulatory sequence , microbiology and biotechnology , genome , gene expression , botany , recombinant dna
A 2.4‐kb pea genomic fragment, containing a member ( rbc S‐E9) of the multigene family encoding the small subunit (rbcS) of ribulose‐1,5‐bisphosphate carboxylase, was inserted into a non‐oncogenic, Ti‐plasmid vector and introduced into the genomes of Petunia hybrida (Mitchell) and Nicotiana tabacum (SR1) plants by in vitro transformation. Petunia and tobacco plants containing the introduced pea rbc S‐E9 gene were regenerated from protoplasts. In these transgenic plants the rbc S‐E9 gene is transcribed accurately using its own promoter and its expression is light‐induced and organ‐specific. A deletion mutant with 352 bp of 5′‐upstream sequence still retains photoinducibility and leaf‐specific expression. Clonal analysis of independent transgenic petunia plants revealed that chromosomal positions in the recipient plant genome affect the quantitative but not qualitative aspects of rbc S‐E9 expression.