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Efficient expression of an Epstein‐Barr nuclear antigen in Drosophila cells transfected with Epstein‐Barr virus DNA.
Author(s) -
Allday M.J.,
Sinclair J.H.,
MacGillivray A.J.,
Sang J.H.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb04029.x
Subject(s) - biology , transfection , epstein–barr virus , microbiology and biotechnology , dna , drosophila melanogaster , schneider 2 cells , virus , clone (java method) , antigen , genetics , virology , cell culture , gene , rna interference , rna
In a search for exogenous promoters which function in cultured Drosophila cells, we have co‐transfected a D. melanogaster cell line with an Epstein‐Barr virus (EBV) cosmid clone which encodes the Epstein‐Barr nuclear antigen (EBNA‐1). Here we report that Drosophila cells containing stably integrated copies of EBNA‐1 encoding DNA synthesise a polypeptide of mol. wt. identical to that of authentic EBNA‐1, which is detectable with EBNA‐positive but not EBNA‐negative human serum. As in EBV‐transformed lymphoblastoid cells, this neo‐antigen is associated with the nucleus of transfected cells suggesting that cellular localisation signals which operate in mammalian cells are also recognised in insect cells.