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Light‐inducible and tissue‐specific expression of a chimaeric gene under control of the 5′‐flanking sequence of a pea chlorophyll a/b ‐binding protein gene
Author(s) -
Simpson June,
Timko Michael P.,
Cashmore Anthony R.,
Schell Jeff,
Van Montagu Marc,
HerreraEstrella Luis
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03995.x
Subject(s) - biology , gene , 5' flanking region , gene expression , genetics , sequence (biology) , microbiology and biotechnology , regulatory sequence , promoter
We have investigated the regulatory functions of the 5′‐flanking sequences of a chlorophyll a/b ‐binding protein gene from Pisum sativum , using the neomycin phosphotransferase (II) activity from Tn 5 as an enzymatic reporter. We show that 0.4 kb of the upstream flanking sequences of this gene are sufficient for both organ‐specific and light‐regulated expression of our chimaeric constructs in transformed tobacco plants. In addition, we show that sequences farther upstream have a significant influence on the level of transcription of these constructions.