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Use of gene transfer and a novel cosmid rescue strategy to isolate transforming sequences.
Author(s) -
Brady G.,
Funk A.,
Mattern J.,
Schütz G.,
Brown R.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03974.x
Subject(s) - cosmid , funk , biology , gene , genetics , art history , art
Mouse Lewis Lung tumor DNA was ligated to a cosmid containing a geneticin (G418)/kanamycin resistance gene and transferred into NIH3T3 cells. Recipient cells were first selected for geneticin resistance and subsequently for their ability to grow as a tumour when injected into nude mice. By repeating this transfection procedure with DNA from resultant tumours, geneticin‐resistant NIH3T3 cells were obtained which were tumorigenic and contained approximately 1‐5 copies of the transferred cosmid. The functional oncogene was cloned by preparing cosmid libraries of third round tumour DNAs, using a cosmid which does not contain a kanamycin resistance gene. Due to the original linkage of the oncogene with the cosmid containing the kanamycin resistance gene, a series of kanamycin‐resistant cosmids were isolated, five of which contained an active oncogene. Subsequent analysis showed that the oncogene present was highly related to the human N‐ras gene. Using a DNA probe from the MLL N‐ras gene, a non‐transforming counterpart was isolated from mouse liver DNA. A comparison between the two N‐ras genes showed that a mutation at the amino acid position corresponding to 61 in the human gene is responsible for transforming activity of the rescued gene.