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The photocycle of the chloride pump halorhodopsin. II: Quantum yields and a kinetic model
Author(s) -
Oesterhelt D.,
Hegemann P.,
Tittor J.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03938.x
Subject(s) - halorhodopsin , biology , kinetic energy , chloride , biophysics , quantum , biochemistry , physics , bacteriorhodopsin , classical mechanics , materials science , quantum mechanics , membrane , metallurgy
The quantum yield of the primary photoreaction of HR 578 was determined as ø green = 0.34±0.02 and that of the photochemical reconversion of HR L 410 into HR 578 as ø blue = 0.01±0.004. From steady‐state illumination and flash‐photolysis experiments, a quantitative description of HR L 410 formation and decay was made leading to a model of the photocycle in isolated, light‐adapted HR. This model satisfies all known facts about HR including its photochromic behaviour.