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Localization of ribosomal protein S2 on the surface of the 30S subunit from Escherichia coli, using monoclonal antibodies.
Author(s) -
SchwedlerBreitenreuter G.,
Lotti M.,
StöfflerMeilicke M.,
Stöffler G.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03899.x
Subject(s) - biology , 30s , escherichia coli , monoclonal antibody , ribosomal protein , protein subunit , microbiology and biotechnology , ribosomal rna , ribosome , bacterial protein , virology , antibody , bacteria , genetics , gene , rna
Protein S2 has been localized on the surface of the 30S subunit of Escherichia coli by immuno‐electron microscopy. The antibody was obtained from a fusion of myeloma cells with spleen cells of mice, which had been immunized with intact 30S ribosomal subunits of E. coli. The binding site of the antibody was on the head of the small subunit, just above the small lobe, in the region where protein S3 has also been localized. S2 is the first ribosomal protein to have been mapped exclusively with monoclonal antibody.