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Suppressor mutations identify box9 as a central nucleotide sequence in the highly ordered structure of intron RNA in yeast mitochondria.
Author(s) -
Holl J.,
Rödel G.,
Schweyen R.J.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03895.x
Subject(s) - biology , intron , genetics , sequence (biology) , rna , mitochondrion , group ii intron , nucleotide , nucleic acid sequence , mutation , yeast , gene , rna splicing
Data presented here lend support to the notion that RNA splicing in yeast mitochondria depends on the formation of hybrid structures involving the well‐conserved intron sequences box9 and box2. Starting with the cis‐dominant splicing‐defective box2 mutant G2590, a G‐‐‐‐A transition, we isolated a revertant having a mitochondrial second site suppressor mutation, which restores splicing competence in the presence of the original mutation. Sequence analysis reveals that the suppressor mutation is a C‐‐‐‐T transition in box9(5′ part). This second mutation compensates for the first one in box2 and restores a box2/box9(5′) hybrid. Combined with previous data demonstrating an interaction of the adjacent sequence box9(3′ part) with the upstream box9c sequence in intron 4, the central role of box9 in the formation of the intron 4, the central role of box9 in the formation of the intron 4 RNA high order structure becomes evident.