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The two intervening sequences of human beta‐ and gamma‐globin pre‐mRNAs are excised in a preferred temporal order in vitro.
Author(s) -
Lang K.M.,
Santen V.L.,
Spritz R.A.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03882.x
Subject(s) - in vitro , globin , physics , order (exchange) , rna splicing , beta (programming language) , microbiology and biotechnology , biology , gene , genetics , computer science , rna , programming language , finance , economics
Splicing of complete synthetic human beta‐ and gamma‐globin mRNA precursors (pre‐mRNAs) follows a preferred pathway in vitro. In almost all molecules, excision of the first intervening sequence (IVS) is completed before excision of the second IVS initiates. However, in a very small fraction of molecules the order of IVS excision is reversed.