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A glia‐derived neurite‐promoting factor with protease inhibitory activity.
Author(s) -
Guenther J.,
Nick H.,
Monard D.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03878.x
Subject(s) - biology , neurite , protease , inhibitory postsynaptic potential , microbiology and biotechnology , neuroscience , biochemistry , enzyme , in vitro
Brain cells and glioma cells in culture release a protein which induces neurite outgrowth in neuroblastoma cells. This neurite‐promoting factor (NPF), which has been purified from serum‐free glioma conditioned medium, has an apparent mol. wt. of 43 000. NPF inhibits urokinase as well as plasminogen activator‐dependent caseinolysis or fibrinolysis. NPF and urokinase form an SDS‐resistant complex. The fact that this glia‐derived NPF is a potent protease inhibitor indicates that glial cells modulate the proteolytic activity associated with neuronal cells and suggests that this phenomenon is one of the biochemical events involved in the regulation of neurite growth.