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Expression of tobacco mosaic virus coat protein by a cauliflower mosaic virus promoter in plants transformed by Agrobacterium
Author(s) -
Bevan M.W.,
Mason S.E.,
Goelet P.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03871.x
Subject(s) - mosaic , biology , tobacco mosaic virus , library science , alfalfa mosaic virus , cauliflower mosaic virus , coat protein , virus , archaeology , virology , geography , genetics , gene , genetically modified crops , rna , transgene , computer science
The 35S transcript promoter of cauliflower mosaic virus directs the synthesis of an abundant RNA in infected leaf tissue that is both a template for reverse transcription during virus replication and also a polycistronic mRNA. The 35S promoter was isolated and linked to a cDNA of the 3′ end of tobacco mosaic virus that encodes the coat protein of this single‐stranded RNA virus. After transformation of this gene construction into tobacco plants using a disarmed Agrobacterium binary vector, viral coat protein synthesis was detected in transformed leaf tissue. A chimaeric 35S coat protein RNA was detected in transformed callus.