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Progenitor cells from embryonic chick dorsal root ganglia differentiate in vitro to neurons: biochemical and electrophysiological evidence.
Author(s) -
Rohrer H.,
HenkeFahle S.,
ElSharkawy T.,
Lux H.D.,
Thoenen H.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03840.x
Subject(s) - biology , embryonic stem cell , electrophysiology , dorsum , anatomy , microbiology and biotechnology , neuroscience , progenitor , in vitro , dorsal root ganglion , progenitor cell , stem cell , genetics , gene
We have analyzed the appearance of neurons and glial cells in chick dorsal root ganglia during development. Neurons were identified by the presence of polysialogangliosides recognized by tetanus toxin (GD1b, GT1) or by the monoclonal antibody Q211 directed against polysialogangliosides containing four, five and six sialic acid residues. Glial cells were identified by the presence of 04 antigen. A population of undifferentiated cells, i.e., cells which express neither neuronal nor glial cell surface antigens, present in dorsal root ganglia until embryonic day 7, was separated from the neuronal and glial population. This cell population contains neuronal progenitor cells which differentiate to neurons within 1 day in culture. This differentiation process is characterized by the appearance of neuronal morphology, of neuron‐specific gangliosides and by the appearance of voltage‐dependent sodium and calcium channels.