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Mutations within the 23S rRNA coding sequence of E. coli which block ribosome assembly.
Author(s) -
Skinner R.H.,
Stark M.J.,
Dahlberg A.E.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03824.x
Subject(s) - 23s ribosomal rna , ribosomal rna , genetics , biology , ribosome , mutagenesis , gene , coding region , mutation , microbiology and biotechnology , rna
We have used site specific mutagenesis in vitro to construct a set of deletion mutations within the 5′ region of a cloned 23S rRNA gene. In contrast to previously studied mutations in this gene, some of these deletions prevent the incorporation of 23S rRNA into ribosomal particles. This result is discussed in terms of a model in which interaction with the assembly initiator protein, L24, is perturbed.