Premium
Tumor promoter phorbol‐12‐myristate‐13‐acetate induces poly(ADP)‐ribosylation in fibroblasts.
Author(s) -
Singh N.,
Poirier G.,
Cerutti P.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03807.x
Subject(s) - phorbol , adp ribosylation , biology , microbiology and biotechnology , chromatin , phorbol ester , dna , biochemistry , phosphorylation , protein kinase c , enzyme , nad+ kinase
The tumor promoter phorbol‐12‐myristate‐13‐acetate (PMA) causes an increase in pol(ADP)‐ribosylation in mouse and human fibroblasts via the intermediate formation of active oxygen. In contrast to poly(ADP)‐ribosylation induced by the methylating agent N‐methyl‐N'‐nitro‐N‐nitrosoguanidine, de novo RNA and protein synthesis are required and the accumulation of the polymer occurs in the absence of detectable DNA strand breakage. Our results suggest a mechanism for PMA‐induced modulation of chromatin structure and gene expression.