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The wheat chloroplast gene for CF 0 subunit I of ATP synthase contains a large intron
Author(s) -
Bird C.R.,
Koller B.,
Auffret A.D.,
Huttly A.K.,
Howe C.J.,
Dyer T.A.,
Gray J.C.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03790.x
Subject(s) - chloroplast , library science , biology , gene , genetics , computer science
The gene for CF 0 subunit I of ATP synthase has been located in wheat chloroplast DNA, between the genes for CF 0 subunit III and α subunit of CF 1 . Nucleotide sequencing and analysis of RNA‐DNA hybrids indicated that the gene is interrupted by an 823‐bp intron which has boundaries similar to those previously described for the introns in protein‐coding chloroplast genes of Euglena gracilis . The deduced amino acid sequence of CF 0 subunit I indicates a polypeptide of 183 amino acid residues. However, N‐terminal amino acid sequencing of the mature spinach CF 0 subunit I suggests that the protein is synthesised with a N‐terminal extension of 17 amino acid residues and is processed to give a protein of mol. wt. 19 001 of 166 amino acids residues. The mature CF 0 subunit I shows similarities in primary and predicted secondary structure to F 0 subunit b of Escherichia coli ATP synthase. A major transcript of 3.3 kb containing sequences from the genes for CF 0 subunit III, subunit I and CF 1 subunit α has been observed by RNA‐DNA hybridisation.