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Chloroplast transformation by Agrobacterium tumefaciens
Author(s) -
De Block Marc,
Schell Jeff,
Van Montagu Marc
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03788.x
Subject(s) - biology , humanities , philosophy
A chimeric gene consisting of the promoter region of the nopaline synthase gene (P nos ) fused to the coding sequence of the chloramphenicol acetyltransferase gene ( cat gene) of Tn9 was introduced by co‐cultivation in tobacco protoplasts followed by selection with 10 μg/ml chloramphenicol. The chloramphenicol‐resistant plants derived from these selected calli were unable to transmit the Cm R phenotype through pollen. A typically maternal inheritance pattern was observed. Southern blot analysis showed that the chimeric P nos‐cat gene was present in the chloroplasts of these resistant plants. Furthermore, the chloramphenicol acetyltransferase activity was shown to be associated with the chloroplast fraction. These observations are the first proof that the Agrobacterium Ti‐plasmid vectors can be used to introduce genes in chloroplasts.