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Cloning an Aspergillus nidulans developmental gene by transformation.
Author(s) -
Johnstone I.L.,
Hughes S.G.,
Clutterbuck A.J.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03777.x
Subject(s) - aspergillus nidulans , biology , transformation (genetics) , cloning (programming) , plasmid , clone (java method) , genetics , gene , computational biology , computer science , programming language , mutant
We have developed a transformation system for Aspergillus nidulans giving a frequency of transformation high enough to screen a gene bank from which we were able to isolate and clone the A. nidulans developmental gene brlA by visual selection. The vector contains the selective marker argB+, and with it a frequency of transformation of 500 stable transformants/micrograms plasmid DNA can regularly be achieved. The evidence suggests that transformation is by integration but spontaneous excision of integrated plasmids is apparently frequent enough to allow the recovery of transforming plasmids in Escherichia coli.