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Nucleotide sequence of the S‐1 mitochondrial DNA from the S cytoplasm of maize
Author(s) -
Paillard Michèle,
Sederoff Ronald R.,
Levings Charles S.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03749.x
Subject(s) - biology , state (computer science) , mitochondrial dna , genetics , library science , gene , mathematics , algorithm , computer science
Mitochondria from the S male‐sterile cytoplasm of maize contain unique DNA‐protein complexes, designated S‐1 and S‐2. These complexes consist of double‐stranded linear DNAs with proteins covalently attached to the 5′ termini. To learn more about these unusual DNAs we have determined the complete nucleotide sequence of the S‐1 DNA molecule (6397 bp). The sequence of S‐2 has been previously determined. S‐1 and S‐2 are structurally similar and contain ˜1.7kb of sequence homology. S‐1 is terminated by exact 208‐bp inverted repeats that are identical with the terminal inverted repeats of S‐2. S‐1 and S‐2 also contain a 1462‐bp region of nearly perfect homology, which includes one of the terminal inverted repeats. The homology between the two molecules may be maintained, in part, by homologous recombination. S‐1 has three long unidentified open reading frames, URF2 (1017 bp), URF3 (2787 bp) and URF4 (768 bp). URF2 occurs in the 1462‐bp region of homology and is identical in length and location in both S‐1 and S‐2. Based on their structural organization and their viral‐like characteristics, we propose that S‐1 and S‐2 code for functions involved with their maintenance and replication.