Premium
A positive regulatory element is involved in the induction of the beta‐galactosidase gene from Kluyveromyces lactis.
Author(s) -
Das S.,
Breunig K.D.,
Hollenberg C.P.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03699.x
Subject(s) - kluyveromyces lactis , biology , kluyveromyces , gene , beta galactosidase , beta (programming language) , regulation of gene expression , saccharomyces cerevisiae , gene expression , genetics , computer science , programming language
The regulation of the LAC4 gene encoding beta‐galactosidase in the yeast Kluyveromyces lactis has been studied. The expression of cloned LAC4 gene present on autonomously replicating plasmids was normally regulated by lactose or galactose as inducers. The LAC4 transcription initiation sites were mapped on two plasmids, PTY75‐LAC4 and pKL2. The sites were found to be dependent on the level of gene expression and on the plasmid used. Under induced conditions, the normal cluster of initiation sites was used on both plasmids, whereas under non‐induced conditions LAC4 on pKL2 showed additional sites. Deletion mapping of the 5′ regulatory region of the LAC4 gene revealed a DNA element required for induction, presumably for the binding of a positive regulator.