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In vivo cloning of the pectate lyase and cellulase genes of Erwinia chrysanthemi
Author(s) -
Gijsegem Frédérique,
Toussaint Ariane,
Schoonejans Eric
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03698.x
Subject(s) - pectate lyase , biology , humanities , cellulase , saint , microbiology and biotechnology , art history , art , biochemistry , enzyme , pectinase
Using an RP4 plasmid which carries a mini‐Mu prophage which allows it to integrate spontaneously random pieces of its host chromosome, we cloned in vivo at least some of the pectate lyase and cellulase genes of the Erwinia chrysanthemi strain B374. The RP4‐prime plasmids were used to localize the cloned genes on the B374 chromosome by co‐transposition mapping and to subclone most of the genes in a classic high copy number plasmid vector.