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dnaA protein‐regulated transcription: effects on the in vitro replication of Escherichia coli minichromosomes.
Author(s) -
Lother H.,
Kölling R.,
Kücherer C.,
Schauzu M.
Publication year - 1985
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1985.tb03664.x
Subject(s) - dnaa , biology , transcription (linguistics) , minichromosome , microbiology and biotechnology , in vitro , escherichia coli , escherichia coli proteins , promoter , origin of replication , dna replication , genetics , gene , gene expression , genome , linguistics , philosophy
Both initiation of replication and initiation of transcription are influenced by dnaA protein, when minichromosomes are assayed in vitro for dnaA protein complementation. This dnaA protein effect is seen only if minichromosomes are used containing the 16‐kd promoter, from which transcription is directed into the minimal origin. Determination of the 16‐kd promoter activity both in vivo and in vitro showed that this strong promoter is specifically repressed by dnaA protein. The 16‐kd promoter is thus an integral regulatory region of oriC.