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Transformation of mammalian fibroblasts and macrophages in vitro by a murine retrovirus encoding an avian v‐myc oncogene.
Author(s) -
Vennström B.,
Kahn P.,
Adkins B.,
Enrietto P.,
Hayman M.J.,
Graf T.,
Luciw P.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb02282.x
Subject(s) - retrovirus , oncogene , biology , transformation (genetics) , virology , virus , gene , genetics , cell cycle
A murine retrovirus which expresses the avain v‐myc OK10 oncogene was constructed. The virus, denoted MMCV, readily transforms fibroblasts of established lines, such as mouse NIH/3T3 and rat 208F cells, to anchorage‐independent growth in agarose. The virus also transforms primary mouse cells: (i) virus‐infected macrophages are induced to form large colonies in semi‐solid media, and can easily be expanded into mass cultures; (ii) MMCV‐infected fibroblastic cells from mouse limb buds undergo morphological transformation and grow in semi‐solid medium. MMCV thus transforms both mouse fibroblastic cells and macrophages in vitro, in a fashion similar to the v‐myc‐containing avian viruses in chicken cells. The possibility of introducing a transforming myc gene into mammalian cells by virus infection provides a novel approach for studying the mechanism of myc transformation in cells from many lineages.