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A putative primer for second‐strand DNA synthesis of maize streak virus is virion‐associated.
Author(s) -
Donson J.,
MorrisKrsinich B.A.,
Mullineaux P.M.,
Boulton M.I.,
Davies J.W.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb02260.x
Subject(s) - biology , primer (cosmetics) , dna , genetics , virology , virus , microbiology and biotechnology , organic chemistry , chemistry
We have isolated, from maize streak virus (MSV) preparations, a population of ‘nested’ DNA molecules. These molecules have ribonucleotides covalently linked to the DNA species’ discrete 5′ deoxyribonucleotide terminus. The major species has a DNA sequence of 80 nucleotides which is complementary to a region 5′ of two hairpin structures on the MSV genome, almost exclusively in an intergenic region. These molecules have been used to prime the synthesis in vitro of a complementary strand to virion DNA, initiating this reaction at one site on the genome.