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Kinetics of internalisation and degradation of surface‐bound interferon in human lymphoblastoid cells.
Author(s) -
Evans T.,
Secher D.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb02243.x
Subject(s) - biology , kinetics , lymphoblast , degradation (telecommunications) , interferon , biophysics , biochemistry , microbiology and biotechnology , virology , cell culture , genetics , physics , quantum mechanics , telecommunications , computer science
The binding of iodinated human interferon‐alpha 2 (IFN‐alpha 2) was studied on the human T cell line, Molt 4. After its initial binding to cells, the IFN is transferred to a trypsin‐resistant compartment before appearing in the medium as TCA‐soluble material, while the total cell‐associated IFN declines to one‐third of its maximum value after 3 h incubation. The Na+/H+ ionophore monensin did not prevent intracellular accumulation of IFN but did completely inhibit its breakdown. We interpret our results as evidence for receptor‐mediated internalisation of IFN followed by intracellular breakdown.