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Histone H4 gene is transcribed in S phase but also late in G 2 phase in Physarum polycephalum
Author(s) -
Wilhelm M.L.,
Toublan B.,
Jalouzot R.,
Wilhelm F.X.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb02190.x
Subject(s) - physarum polycephalum , biology , physarum , histone , genetics , gene , phase (matter) , microbiology and biotechnology , physics , quantum mechanics
The myxomycete Physarum polycephalum contains two types of H4 histone genes. Southern blotting of restriction endonuclease fragments of P. polycephalum DNA and hybridization to a cloned probe labelled by nick‐translation indicate that there are only one or two copies of each H4 gene per haploid genome. A cloned homologous genomic probe was used to study the cellular abundance of H4 mRNA during the cell cycle. We report that the H4 mRNA is not only transcribed in S phase as previously described for other organisms but that transcription of the H4 gene also occurs at the end of G 2 phase. Since no translation of the histone messenger was observed in G 2 phase this suggests that the histone mRNA synthesized in G 2 constitutes a pool of molecules in anticipation of the next S phase.