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Encephalomyocarditis virus RNA synthesis in vitro is protein‐primed.
Author(s) -
Vartapetian A.B.,
Koonin E.V.,
Agol V.I.,
Bogdanov A.A.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb02179.x
Subject(s) - biology , rna , primer (cosmetics) , in vitro , virus , oligonucleotide , virology , microbiology and biotechnology , computational biology , genetics , dna , gene , physics , thermodynamics
A crude membrane‐bound replication complex isolated from encephalomyocarditis virus‐infected cells is able to initiate the synthesis of viral RNA. Both the formation of the primer, VPg‐pU, and its utilization for the initiation of RNA chains take place in this system. A significant amount of the synthesized VPg‐pU is found in the free form. The predominant product of the de novo initiation is represented by short phenol‐soluble VPg oligonucleotide species, and only a small percentage of the latter appear to be elongated into longer RNA chains.