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Delivery of T‐DNA from the Agrobacterium tumefaciens chromosome into plant cells
Author(s) -
Hoekema A.,
Roelvink P.W.,
Hooykaas P.J.J.,
Schilperoort R.A.
Publication year - 1984
Publication title -
the embo journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 7.484
H-Index - 392
eISSN - 1460-2075
pISSN - 0261-4189
DOI - 10.1002/j.1460-2075.1984.tb02160.x
Subject(s) - biology , agrobacterium tumefaciens , dna , plant cell , genetics , ti plasmid , agrobacterium , transfer dna , rhizobiaceae , botany , chromosome , microbiology and biotechnology , transformation (genetics) , gene , bacteria , symbiosis
The intact T‐region of the B6Ti plasmid of Agrobacterium tumefaciens was stepwise cloned into a site in transposon Tn3. In this way a suitable vehicle (Tn1882) was obtained for translocating the T‐region to different replicons, i.e., to other plasmids or the chromosome. The IncP plasmid R772::Tn1882 conferred tumorigenicity on Agrobacterium if the virulence genes were provided in trans in the same cell. This result showed that the T‐region present on Tn1882 was transferred efficiently to plant cells. Normal tumor development also occurred if the T‐region was placed in the chromosome of A. tumefaciens and an R’ plasmid was present carrying vir A–E or vir A–F. We conclude that the plasmid location of the T‐region is not a prerequisite for transfer to the plant cell. The apparently normal delivery of the T‐DNA from a bacterial chromosomal location supports a model involving a processing step within Agrobacterium effecting transfer of the T‐region as a separate entity.